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These movies are confocal time series showing early steps in the infection of amoebae of Dictyostelium discoideum by the bacterial pathogen Legionella pneumophila, the causative agent of Legionnaires’ disease. These movies appeared in Lu, H., and Clarke, M., Cell. Microbiol. 7, 995-1007 (2005), and are included here with permission of Blackwell Publishing.
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Movie
1.
Lu_and_Clarke_1.mov,
320x240 pixels, 1.7MB compressed
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Phagocytosis of L. pneumophila by a Dictyostelium amoeba. The Dictyostelium cell is expressing GFP fused to the actin-binding protein coronin, and the bacteria are expressing with DsRed Express. Coronin-GFP surrounds the nascent phagosome, but that coat is shed directly after the phagosome is internalized. This time series spans 146 seconds; the movie plays about 13 times faster.
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Movie
2.
Lu_and_Clarke_2.mov,
335x327 pixels, 1.5MB compressed
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A Legionella-containing phagosome undergoing microtubule based transport. The microtubules are labeled with GFP-α-tubulin. The movie shows a phagosome containing wild-type L. pneumophila undergoing intermittent association with and movement along microtubules in a Dictyostelium cell. The movie (spanning 105 seconds) plays about 13 times faster than the actual rate.
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Movie
3.
Lu_and_Clarke_3.mov,
494.344 pixels, 1.7MB compressed
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Association of ER membrane with Legionella-containing phagosomes. The Dictyostelium cells are expressing calnexin-GFP, a trans-membrane resident of the endoplasmic reticulum. This marker is not initially associated with Legionella-containing phagosomes, but begins to accumulate about 1 hour after uptake, correlating with diminished phagosome mobility. These cells were mixed with wild-type L. pneumophila two hours earlier. The upper bacterium in the right-hand cell is moving rapidly about the cell, indicating that it was ingested recently. A second bacterium in the same cell is undergoing shorter and less frequent movements; it is initially free of calnexin-GFP but has begun to acquire this marker by the end of the time series. The left-hand cell contains three phagosomes (probably ingested ~ 2 hours ago) that are strongly labeled with calnexin-GFP; their motion is restricted to that of the contiguous ER. The movie plays at about 13 times the actual rate.
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| last
updated:
November 21, 2005
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